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Appropriate homologs were selected based on best e-value and greatest similarity.
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Sequences (>50 bp) were assigned on the basis of best E-value BLASTN scores (http://blast.ncbi.nlm.nih.gov/blast.cgi) in GenBank.
Next, a representative gene model sequence for each gene cluster was selected based on best E value matches, sequence identity and coverage of nr proteins (non-redundant proteins of NCBI).
The functional classification of all ESTs was established based on the database entry giving the best E-value and the categories defined by Bevan et al. [ 25].
The functional classification of all ESTs was established based on the database entry giving the best E-value using the Bevan's classification categories.
We considered 14 bacterial genomes, and for each of them, based on the ROC curve analysis, we determined the best e-value threshold for BLAST [ 17] and the best model organism to use, between Bacillus subtilis and E. coli, to map regulatory information.
Each sequence is finally assigned to the subfamily which shows the best e-value.
The networks in this work use the best E-value associated with each pairwise comparison.
The precise value of T, on the other hand, is not critical for a similar reason; most Blast hits are found to be either very close to the best Blast hit (usually with an E-value within a factor of 100 or so of the best E-value) or considerably further removed.
Contigs with a best e-value of 10-25 or lower were assigned a putative identity.
The number of contigs and the best e-value obtained are given.
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