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That's an appalling tackle – much worse than Gascoigne's on Berthold – and he is rightly booked.
Luciferase assay were performed using the Dual-luciferase Reporter Assay System (Promega) following the manufacture's instruction, on BERTHOLD TriStar LB 941 (BERTHOLD TECHNOLOGIES, Bad Wildbad, Germany) The data were presented as mean ± standard deviation (S.D .. IC50 is the median growth inhibitory concentration value, calculated using GraphPad Prism 5.0 software (La Jolla, CA).
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This same Neoplatonic tradition also inspired, however, a major critique of the doctrine of the transcendentals, as formulated in the commentary of Berthold of Moosburg on the Elementatio theologica of Proclus and in the works of Nicolas of Cusa.
After a 21-day ingrowth period, the source was counted for gross α-activity on a Berthold LB770 low-level proportional counter (LB 770 10-Channel α-β-low-level counter, Berthold Technologies GmbH & Co). for 1000 min.
The plate was read on a Berthold MicroLumat Plus LB 96V (Germany) using WinGlow software (Berthold).
Luminometry was performed on a Berthold Technologies Lumat 9507 (Willbad, Germany).
Firefly luciferase activities were measured on a Berthold Lumat LB 9507 luminometer.
FF and Ren luc activity (RLU) were measured on a Berthold Lumat LB 9507 luminometer.
The luciferase activity was measured by the addition of 20 µL of lysate to 100 µL of luciferase assay reagent II (Promega) in a 5 mL test tube followed by reading on a Berthold Lumat LB9501 luminometer (Berthold Technologies).
D-Luciferin (Caliper Life Sciences, Alameda, CA; 200 μg/ml) was added and luminescence was detected on a Berthold LB 940 Mirthras.
Lysates were harvested 24 h post transfection, and luciferase measured according to the dual luciferase protocol (Promega) on a Berthold luminometer (Berthold, UK).
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