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The selectivity of the vesicles was measured via a simple fluorescence "switch on" assay.
To provide data on assay specificity, samples from patients with other rheumatic diseases (nonRA) were also analyzed.
Together, these findings indicate that a one-step assay format can greatly reduce the effect of coating concentration variation on assay performance.
On assay plates with rif, mean population density varied from nearly 102 to nearly 1010.
The formulations were assessed on assay, dissolution, friability, weight variation and disintegration time.
Data analysis methods had a pronounced impact on assay sensitivity and specificity calculations.
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In this study, a new type of rapid, label-free fluorescence turn-on assay for detection of tiopronin using Alizarin Red S (ARS)/copper ion ensemble is developed.
The nuclear run-on assay has been previously described [44].
Nuclear run-on assay was performed following the previous protocol [94] with little modification.
We report here the global measurement of transcription by adapting the nuclear run-on assay to commercial microarrays.
We performed a nuclear run-on assay using the low concentrations (20 µg/ml) of α-amanitin that inhibit pol II but not pol I and III.
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