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At least one PCR NTC was run for each assay as controls on all plates.
Conversely, Colletotrichum acutatum showed significant differences in growth until 35 days when the isolate grew to the edge of the Petri dish on all plates.
We used several standard controls on all plates that were genotyped, including the reference genome line Nipponbare, 93-11, an F1 between Nipponbare and 93-11 (kindly supplied by Guo-liang Wang of Ohio State University) and a "pseudo F1" that was prepared by mixing equal amounts of genomic DNA from Nipponbare and 93-11.
On all plates, PBS was included as a non-inhibitory control and 1F9 anti-AMA1 monoclonal antibody (0.5 mg/ml) [39] was included as an inhibitory control.
However, nematodes fed E. coli avoided these strains after a brief learning period and by 48 h the nematode populations grew on all plates.
Since food was still present on all plates after 10 days, they were transferred to 19.5°C for suppressor candidates to produce progeny.
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Pentamidine was placed on row G of all plates as a control.
A single experiment consists of all plates transfected on the same day.
On all the plates".
The worms on all these plates were scored for survival every 24 hrs.
Presence of background lawn on all the plates was confirmed.
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