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Briefly, moss tissues were grown at 25°C under the cycle of 14 h light (90 μmol m-2 s-1) and 10 h dark for one week on a thin layer of solid PpNO3 medium in glass bottom dishes.
If for some reason you can't follow the standard steps, consider one of the following ideas: Try spreading on a thin layer of virgin coconut oil and zinc oxide.
The man stood, very still, on a thin layer of gray slime.
Meanwhile, ladle on a thin layer of tomato sauce, leaving 2.5cm around the edge for the crust to rise.
Zinsou is talking about how the city is plagued by flooding, because it lies below sea level and is built on a thin layer of sand.
Finally, a second photograph of another tree was printed on a thin layer of paper that was saturated with varnish and then laid over the existing image.
And the three elephants -- Jan, Bunny and Marie -- rocked themselves back and forth and then lay down on a thin layer of straw for a nap.
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The reactants were spotted on a thin-layer chromatography (TLC) silica gel 60 plate (Merck, Darmstadt, Germany) and developed twice in an acetonitrile water (85:15, v/v) solution.
The phases were separated by centrifugation at 2000 g for 2 min, and the organic phase was spotted on a thin-layer chromatography plate (EMD Kieselgel 60, VWR).
The lipids were extracted with 160 µl of chloroform methanol (1 1), spotted on a thin-layer chromatography plate, and separated with chloroform-acetone-methanol-glacial acetic acid-H2O (40 15 13 12 8).
The lipids were extracted with 160 μL of a chloroform/methanol mixture (1 1), spotted on a thin-layer chromatography plate, and separated with a chloroform/acetone/methanol/glacial acetic acid/H2O mixture (40 15 13 12 8).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com