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A 1 ml sample of the bacteria solution was aliquoted on to a plate, nutrient agar was added and the combination was mixed well.
Briefly, frozen preserved stock was thawed at room temperature, and then 0.1 mL were pipetted and streaked into a quadrant on a nutrient agar plate.
For each of the test tube in the MIC determination that showed a visible growth, a loopful of the broth was inoculated on a sterile nutrient agar plates using agar streaked method.
On the third day, oat flakes were added directly onto an actively growing plasmodium on a nutrient agar plate and incubated for one additional day.
To extract DNA a sweep of growth on a nutrient agar slant were boiled in 500 μL of sterile distilled water for 10 minutes.
Colonies to be screened were picked and arrayed on Hybond-N membranes (Amersham Biosciences, Buckinghamshire UK) that were sitting on a surface of nutrient agar [ 110].
The bacteria were grown on a nutrient agar surface at 7 °C.
Streptomyces clavuligerus was streaked on a nutrient agar plate.
The microorganisms were cultured on a nutrient agar plate and incubated at 37 °C for 24 h.
The diluted solution was plated on a nutrient agar and incubated for 24 h at 37°C ± 2°C.
After vigorous stirring (2,500 rpm for 1 min), the solution in each jar was poured on a nutrient agar plate.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com