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An identity match of 98% was selected to allow one or two mismatches on a single sequence.
This technique is based on a single sequence analysis of several intergenic regions selected by complete genome sequence comparison, resulting in a sequencing-based, genotypic profile [22].
Thus far our description was based on a single sequence.
The procedure continued until the consensus converged on a single sequence.
For the subfamilies CYP1A, CYP2A and CYP2E, primers were designed based on a single sequence.
For each virus, we compared the predictions of multiple programs on a single sequence ("vertical approach"), using ANNIE [ 101].
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All cDNA library preparation protocols currently available provide the utilization of the entire library for a single sequencing run on a single dedicated platform.
We sequenced Palmer Station LTER amplicons on a single sequencing run, with the eukaryotic amplicons comprising approximately 25%, or 325,000, of the 1,500,000 beads loaded on the PicoTiterPlate (Roche, Basel, Switzerland).
Samples from 12 tissues could be displayed on a single sequencing gel; thus we could effectively screen all known human PTKs in a short period of time.
Samples from six human gastric cancer cell lines could be displayed on a single sequencing gel; thus, we could effectively screen all known human PTKs with only a few gel displays.
"We can't really look at DNA sequences yet, or at least not a single sequence on its own.
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