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Neutrophils were isolated immediately after blood sampling by sedimentation on a separating medium containing 5% Dextran T500® (Pharmacia, Uppsala, Sweden) in 0.9% saline and centrifugation on a Ficoll gradient (Eurobio, Les Ulis, France) as previously described [26, 27].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com