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We cloned the gene for cytochrome c7 and expressed it in Escherichiacoli together with the cytochrome c maturation gene cluster, ccmABCDEFGH, on a separate plasmid.
The target DNA regions were harbored on a separate plasmid.
The recombinase genes were harbored on a separate plasmid from the switch to facilitate testing.
This vector has a self-inactivating LTR and envelope, VsVg, is expressed on a separate plasmid [51].
In contrast, neither did an N-terminal fusion of GST to the NTD nor the expression of GST in trans to the NTD (on a separate plasmid co-transformed with the NTD-pET plasmid) yield any protein in the soluble fraction.
Copy numbers of methylated and unmethylated sequences were quantified based on a separate plasmid standard curve for each.
Similar(49)
The relative expression of CAT from these plasmids was examined in the absence and presence of a separate plasmid expressing the CaMV TAV protein (pHELP7) [23].
Each gene was cloned into a separate plasmid.
GFP was expressed from a separate plasmid as a transfection marker.
K562 cells were nucleofected with both WAS-specific ZFNs, each expressed from a separate plasmid, and a third plasmid encoding the donor template DNA (Fig. 1A).
Alternatively the Cre can be expressed from a separate plasmid that is co-transfected with the retrofitting plasmid, and then lost once retrofitting has occurred.
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