Too late to hold on to a nap?
On his return, he settled on a couch for a nap.
If any units did go out, they went no farther than the nearest canal for a nap on the bank.
Maybe when she's put him down for a nap on his bed of swansdown and fairy wings".
By using (4) and (5), we newly define and as the current bandwidth loaded on an nAP and its busy ratio, respectively, when the nAP has active STAs.
The labeling was terminated by purification on a NAP-5 column (Ge Healthcare) equilibrated with PBS (Sigma Aldrich).
The 99mTc-nanobody solution was purified on a NAP-5 column (GE Healthcare, Little Chalfont, Buckinghamshire, UK) pre-equilibrated with PBS.
After 2 h incubation (3 h for 5 compounds to yield sufficient labelling efficiency), the product was purified on a NAP-5 column (GE Healthcare).
Each labelled protein was separated from non-reacted 125I and low-molecular-weight reaction components on a NAP-5 column pre-equilibrated with PBS.
The fragment used for 111In-labelling was separated by size-exclusion chromatography on a NAP-5 column (Amersham Biosciences, Uppsala, Sweden) pre-equilibrated with purified (MilliQ, Millipore Corp., Billerica, MA, USA) water.
For radiolabelling, approximately 125 μL of DTPA-11B6 (approximately 1 μg/μL in 0.2 M ammonium acetate buffer pH 5.5) was mixed with a predetermined amount (approximately 50 to 100 MBq) of 111InCl3 (Mallinckrodt Medical; Dublin, Ireland), incubated at room temperature for 1.5 to 2 h and then purified on a NAP-5 column (GE Healthcare) equilibrated with PBS Thermo Scientific WalthamaMA MA, USA).
