Exact(2)
To confirm that these are indeed galK+ and to make sure that single colonies are isolated, streak out 4 6 colonies on a MacConkey indicator plate with galactose and grow over night at 32°C.
Bacteria were grown overnight on a MacConkey agar plates at 35°C.
Similar(6)
Specifically, 0.1 ml each of 100, 10 1, 10 2, 10 3, 10 4 and 10 5 dilutions were taken aseptically under the laminar flow hood and inoculated on a solidified MacConkey (Oxoid Ltd., Basingstoke and Hampshire, England), Salmonella-Shigella (Techno Pharmchem, India) and Mannitol Egg Yolk Polymyxin (MYP) agars.
A 1oopful of the content of each test tube was inoculated by streaking on a solidified MacConkey agar plate and then incubated at 37°C for 24 hours for possible bacterial growth.
The following day, the bacteria suspension was tested for purity on two non-selective enriched agars: mastitis blood agar plates (BA; Steins Eurofins A/S, Holstebro, Denmark) and tryptic soya agar (TSA; BD Difco™ - BD Denmand A/S) and for E. coli on a selective coliform MacConkey agar (MCA; Merck).
Results of biochemical tests of the isolate were positive for Mycobacterium spp. by a 2-week culture on MacConkey agar and a heat catalase test but weakly positive by 3-day arylsulfatase and 2-week catalase testing (8 ).
CRKP screening of patients was performed by plating rectal swabs on selective culture media containing cefpodoxime (ChromID ESBL, bioMerieux) and on MacConkey plates on which an ertapenem disk (10 μg) was placed.
The bacterium after growth was maintained on an agar plate (Macconkey agar) at 4°C temperature.
Related(1)
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