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jThese same techniques, based on a MAB model, prove to be efficient to tackle some DSA related problems as already discussed in Section 3.2.
In Section 5.2 an OSA scenario based on a MAB model, described in article [48], is summarized and illustrates the impact of observation errors on decision making for CR.
A blocking-ELISA based on a mAb against influenza A nucleoprotein (NP) has been reported as quicker and more sensitive than HI for detection of influenza A virus infection [27].
We now report on the localization of this epitope on the B-C loop of FNIII 8. We previously reported on a mAb, C6, which reacts with an epitope within the FNIII 8 that is hindered when the ED-B is omitted but available when the ED-B is inserted within the molecule.
AP was first detected on a mAb by Johnson et al. [ 14], who showed no effect on Fab binding and Fc effector function.
Even different clones of a particular cell line may impart subtle changes on a mAb molecule, and only the original mAb-encoding DNA clone and master cell bank can be guaranteed to generate a consistent product, provided culture conditions are carefully maintained.
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This general relationship has also been validated more recently in humans with the M252Y/S254T/T256E (YTE) set of mutations constructed on the Fc of a mAb against respiratory syncytial virus (RSV) with half-life increases from around 20 days to more than 60 days (Robbie et al., 2013).
We propose using both screens in a complementary fashion to map the binding surface epitope of a mAb on a target cell-surface receptor.
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