The slides were then stained by haemotoxylin and eosin (H&E) dye and mounted on a light microscope for observation.
Twenty root segments were fixed in anhydrous ethanol for above 24 h, and then freehand sections were observed and photographed on a light microscope equipped with a camera (Leica DM 2500, Germany).
Cell lysis was confirmed visually on a light microscope.
Stained larvae were then mounted in glycerol and visualized on a light microscope.
Briefly, trays of ependymal cells were placed in a humidified (80 to 90% humidity) thermostatically controlled (37°C) incubation chamber on a light microscope stage (Diphot; Nikon).
Digital light microscopy (LM) images of stained sections were captured with a Cool Snap Pro color video camera (Media Cybernetics) mounted on a light microscope (Leica) (x40 and x100 magnification) using ImagePro Plus image analysis software (version 4.5.1; Media Cybernetics).
After further rinsing in PBS, the sections were mounted in Fluorosave (Calbiochem, San Diego, CA, USA) and were examined under a confocal laser scanning microscope (Bio-Rad, Hemel Hemstead, UK) equipped with an argon/krypton gas laser mounted on a light microscope (Eclipse E 600; Nikon, Tokio, Japan).
Images were photographed on a light microscope (Leica, Wetzlar, Germany).
The number of adherent erythrocytes was counted on a light microscope.
Sections were imaged on a light microscope (Axioplan II, Carl Zeiss, North Ryde, NSW, Australia).
Sections were counterstained with haematoxylin (BDH), dehydrated and analysed on a light microscope.
