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The AS1-AS2 complex interacts with PRC2 components and the AS1-AS2 binding site from the BP promoter is sufficient to confer Polycomb repression on a GUS transgene.
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The marriage effectively ends on a visit to Gus at Six Mile Bottom, her appropriately named estate.
Gordon cut a path of her own, too, writing for Artforum, curating exhibits, and showing her own artwork; debuting as a producer, at Courtney Love's request, on Hole's critically acclaimed first album, Pretty on the Inside; working on a fashion line; and making guest appearances on everything from a Gus Van Sant film to an episode of Girls.
Hit identification was based on results from a GUS enzyme assay only; however, for a measure of cell growth, 10% (vol/vol) alamarBlue was also added to the cells, and fluorescence was measured at λex of 530 nm and λem of 590 nm after a 4-h incubation (30).
They tried to persuade me to put on a suit, dress as Gus, and wander past the glass behind the newsreader during a live broadcast.
There's an equally flawed logic going on with Gus Van Sant, who makes Spout's hall of shame for his 2000 highschool melodrama Finding Forrester.
The chain was then sold on to GUS, a former incarnation of Home Retail Group, in 2002.
"Unless diversity is walking down your street on a Saturday night," Gus remarks.
Analyzing the arcs and spaces that an untrained eye might not catch on Brooke Astor's signature on a 2004 document, Gus R. Lesnevich came to an unequivocal conclusion.
The only shame was that Dennis Tueart, Jimmy Montgomery, Micky Horswill and friends could not remain on the pitch on a day when Gus Poyet's class of 2014-15 rathuffedffed and puffed into the fourth round.
On a chance encounter, Gus and Carrington, Martin shows Gemma the chateau, just a charming old ruin.
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