Sentence examples for on a double selection from inspiring English sources

As expected, prestin/NubG-Alg5 did not grow on the quadruple dropout selective medium (SD-LTHA, Figure 2B), although both prestin and Alg5 were co-expressed by yeast as demonstrated on a double selection plate (SD-LT).

We recently isolated a novel human anti-ErbB2 single-chain variable fragment (scFv) from a large phage-display library (Griffin 1) through a double selection strategy performed on live cells (De Lorenzo et al, 2002).

The authors suggest the selection of a smaller set of variables using a "double selection method".

Wild-type strain (FY3027 h+) was plated either onto medium lacking arginine (-Arg) and then onto medium lacking uracil (-Ura) or directly on to double selection medium lacking both arginine and uracil (-Arg, -Ura) and mutagenised by UV (3 5 mJ, 50 80% killing).

Resistant seedlings were grown to maturity and progeny of these plants (T2) were grown on double selection again.

Progeny (T1) was grown on double selection: sulfadiazine for selection of the T-DNA insert in the DAYSLEEPER gene and PPT for selection of the construct with the shortened DAYSLEEPER gene.

Amongst the T2 progeny grown on double selection were in all cases (for each construct 8 or 9 independent lines), still plantlets with the daysleeper phenotype (Additional file 2: Data Sheet S1).

A complete double selection was planned if the 10% random sample revealed relevant inconsistencies.

A complete double selection was planned if the 10% random sample assessment revealed relevant inconsistencies.

Then pSET::C was electrotransformed into the ΔsalKR mutant to screen the complemented strain of ΔsalKR (namely CΔsalKR) on THB agar with double selection pressure of Spc and Cm.

The BY-2-TetR line was super-transformed with pTRAhDsRed-eTXvIL-10 and over a hundred independent callus clones were derived by double selection on medium containing kanamycin and hygromycin.