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In batch cultures, the maximum specific growth rate of SCKK006 on glucose in a defined minimal medium with 20 g/L glucose as carbon source was 0.34 h-1.
These values were obtained in a defined minimal medium with a prototrophic and plasmid-free strain, making this process highly interesting for industrial application.
The first report of Mn accumulation in D. radiodurans was by Leibowitz and colleagues [39], who demonstrated that D. radiodurans contained approximately 100 times more Mn than E. coli when grown in a defined minimal medium (DMM).
In this study, we report on transcriptomic analyses of P. aeruginosa PAO1 wild type and parS and parR mutants growing in a defined minimal medium.
Malbranchea pulchella was cultured in a defined minimal medium (MM) [ 29] supplemented with 1% glucose and 0.1% yeast extract at 45°C at 180 rpm. A. nidulans was cultured in complete medium (CM) as previously described [ 30].
A defined minimal medium for C. difficile growth (CDMM), described previously12,49, was used for selection of pyrE mutants by supplementation with 5-fluoroorotic acid (FOA; 2 mg/mL) and uracil (5 µg/mL).
Basically, growth of wild-type strains of A. baylyi was monitored in PM1 and PM2 microplates containing a defined minimal medium supplemented with 190 distinct carbon sources.
In short, defined minimal medium with 4.5 g/L α- D -glucose was used in A-stat experiments.
Wild-type MG1655 and hemA cells were grown in hemA defined minimal medium as described earlier.
B. licheniformis DSM13 was cultivated at 37°C and 160 rpm in a 5 L Erlenmeyer flask on defined minimal medium [ 87].
Here, S. oneidensis MR-1 was cultured in a chemically defined minimal medium as described previously [11].
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