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Non-denaturing SDS-PAGE analysis with equal protein loadings of culture filtrates on a CMC gel from the UV-treated cells showed maximum zone of clearance with the SGUV30 strain (Fig. 3).
Fig. 6 Activity of F1 (in heated, 65 °C, 20 min, crude cell extract) on a CMC and b Avicel as a substrate, detected as fluorescence by the Amplex Ultra Red based on H2O2 formed by glucose oxidase from enzyme reactions generating glucose.
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After electrophoresis, the gel was equilibrated with sodium citrate buffer (50 mM, pH 5.0) for 30 min and was overlayered on a CMC-containing polyacrylamide gel.
Tensile and creeps tests at 1100 °C on a ±45° CMC composed of NextelTM720 fibers and a alumina silica matrix (AS-0) have been performed.
Visual stimuli were presented on a Daewoo CMC-2100 ME, 21 inches color monitor (100 Hz non-interlaced refresh, 640×487 resolution) in the initial experiments and on a 22 inches, Mitsubishi Diamond Pro 2070SB color monitor (100 Hz non-interlaced refresh, 800×600 resolution) in the last experiments.
Hydrolytic activity detected on: (CMC) 1% (w/v) carboxymethyl cellulose agar.
A 0.5-mL culture with a 106 dilution (~105 106 spores mL−1) was spread plated on a 0.5% CMC agar plate under sterile conditions.
Tests were also conducted on carboxymethyl cellulose (CMC), a commonly used mud thickener.
The crude enzyme extract showed activity on carboxymethyl cellulose (CMC), a linear β- 1,4) polymer of D-glucose.
As such, PV automaticity together with elimination of all PV potentials on the CMC seems a definite proof of LA PV entry block.
The present study suggests that entry block (i.e. complete elimination of PVP obtained on the CMC) is a sufficient endpoint for PVI.
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