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In objects whose dimensions exceed the SPECT spatial resolution by several times, quantification based on a calibration procedure similar to the one used in PET seems to be feasible within 10% error limits and even below if a fine-tuning of all acquisition and reconstruction parameters is performed.
Numerical algorithms to select the optimal weight parameters are provided based on a calibration sample.
The determinations were performed in triplicate, and the calculations were based on a calibration curve obtained with pyrogallol.
The determinations were performed in triplicate, and the calculations were based on a calibration curve obtained with quercetin.
The concentration of the methylene blue was calculated based on a calibration curve of the absorbance values versus dye concentrations.
The measurements were performed in triplicate, and the calculations were based on a calibration curve obtained with glucose.
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The phosphorothioate oligonucleotides were quantified on the basis of a calibration curve prepared with six calibration standards of different concentrations.
Thrombin activity was calculated on the basis of a calibration curve of the thrombin activity ratio of 0.1 4 IU/mL.
Based on such a calibration, the mass of ENPs within one organism section was calculated and used to estimate the total mass of ENPs per organism.
To overcome this problem, the approach presented here consists on performing a calibration of such parameters.
A calibration on the basis of identical cell populations measured by both approaches connects the two techniques.
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