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Interestingly, no IgG-band was visible on a blot when cells were treated with EndoS, suggesting a total IgG dissociation from the cells (Fig. 7A).
To identify the truncated proteins that displayed a significant affinity for actin, a far Western assay was performed on a blot of Ciha-1 cellular proteins overlaid with individual truncated PGK proteins.
If a signal is seen on a blot, it could arise from any protein migrating at that position, but mass spectrometry detects only the most abundant ones, which may not react with the sera at all.
The signal of each sample on a blot was normalized to the average signal from samples of NR (for NR and DR comparison) or WT (for WT and mutant comparison) group to obtain the % of average NR or % of average WT values, which were compared across different experimental groups using unpaired Student's t-test.
The amount of heterochromatic repeat in each accession relative to the single standard was calculated as the ratio of the accession's mean value (A) on a blot divided by the standard's (B).
Prior to use in pull-down experiments, extracts of each worm were probed with an anti- B. pahangi Hsp-90 antibody or with AC88, a monoclonal antibody to Hsp-90, to confirm that Hsp-90 could be detected on a blot.
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How much data can you cram on an ink blot, after all?
How many data can you cram on an ink blot, after all?
Later, fetal lungs were isolated en bloc and placed on a blotting paper to remove blood.
The PVDF membrane was charged in 100% methanol for 30 seconds prior to the transfer on a Blot-Module for the XCell II Mini-Cell (Invitrogen).
For aminoterminal sequencing of the salivary proteins, 35 homogenised pairs of salivary glands were electrophoresed and transferred to polyvinylidene difluoride (PVDF) membrane using NuPAGE transfer buffer, 10% methanol as the transfer buffer on a Blot-Module for the XCell II Mini-Cell (Invitrogen).
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