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Control experiments omitting the enzyme were also performed.
A control reaction omitting the enzyme was carried out in parallel.
To reduce the biocatalyst cost by omitting the enzyme purification step, the crude E. coli cell-free extracts of Tll and OleTJE were applied for the production of 1-alkene using microalgae oil as feedstock.
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For the controls, we omitted the enzyme, 2-aminofluorene (2-AF), or PNPA.
Templates created omitting the RT enzyme were included in the RT-PCR in each case.
For control slides, TUNEL Label only was used, omitting the TUNEL enzyme in the reaction mixture, and no staining was detected.
Negative controls were obtained by omitting the TdT enzyme.
Controls were performed by omitting the reverse transcriptase enzyme.
Controls with this kit and similar preparations were performed by omitting the terminal deoxynucleotidyl transferase enzyme from the labeling solution (negative control) and by pretreating the sections for 30 min with DNAse I (Roche, 3 U/ml) in 50 mM Tris-HCl, pH 7.5, 1 mg/ml BSA to induce DNA strand breaks (positive control).
Coverslips were dipped into liquid nitrogen and removed as quickly as possible (less than 1 second) and abalone gut extract was omitted from the enzyme mixture.
Negative controls included omitting the primary antibody.
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