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Samples were analyzed on a FluoStar Omega plate reader for absorbance (600 nm) and DsRed fluorescence (544/590 nm).
Absorbance was spectrophotometrically measured at 562 nm in a FluoStar Omega plate reader (BMG Labtech).
A POLARstar Omega Plate Reader (BMG Labtech) was used to measure the fluorescence changes.
A FluoroStar Omega plate reader (BMG Biosciences) was used for fluorescent detection.
A FLUOstar Omega plate reader (BMG LABTECH® Offenburg Germany) with KlusterCaller™ software was used to visualize KASP marker polymorphisms.
Absorbance at 490 nm (with 650 nm as reference) was measured on an Omega Plate reader (BMG LabTech).
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The plate was read on a FLUOstar-Omega plate reader (Ortenberg, Germany).
Absorbance was measured at 460 nm on a FLUOstar Omega (BMG Labtech) plate reader.
Growth was monitored at 600 nm over 36 h by a FLOUstar omega multiwell plate reader (BMG Labtech, Bucks, UK).
Cell death and caspase-3 like activity analysis using the Fluostar Omega fluorescent plate reader (BMG Labtech GmbH, Ortenberg, Germany) was performed as previously described.
A set of 96-well microtitre plates containing 200 μl YNB supplemented with 100 mM glucose and 1.5 mg ml−1, 2.0 or 3.0 mg ml−1 furfural (Sigma Aldrich, Gillingham, UK) were subsequently inoculated with a 5% volume of each overnight culture and growth was monitored using a FLOUstar omega multiwell plate reader.
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