To explore interactions between respiration, inhibition, and olfaction, experiments using light to active channel rhodopsin in sensory neurons expressing Olfactory Marker Protein were performed in mice and modeled in silico.
Immature neurons arising from cell division express GAP 43 and doublecortin; they differentiate to fully mature neurons expressing OMP (Olfactory Marker Protein) [24] and olfactory receptors at the tip of their dendrite, when establishing synapses with the apical dendrite of mitral/tufted cells in the OB.
Note that Olfactory Marker Protein (OMP) was expressed in mouse but not in human olfactory bulb.
As a trivial illustration, look more closely at the definition Reflect gives to OMP in Figure 8 – Olfactory Marker Protein.
The primary antibodies applied in immunofluorescence staining are: Goat anti-Olfactory Marker Protein (1∶100, Waco Chemicals, USA); Mouse anti-N-cadherin (1∶50, Sigma-Aldrich); Goat anti-ZO-1 (1∶100, Santa Cruz Biotechnology); Goat anti-β-catenin (1∶100, Santa Cruz Biotechnology) and Rabbit anti-N.
The odorant receptor-dependent role of olfactory marker protein in olfactory receptor neurons.
To avoid double counting, each olfactory glomerulus was marked using ImageJ including the Cell counter plugin (De Vos, K., University of Sheffield).
Loss of OMP-stained olfactory nerves was most marked in the lateral and medial aspects of each of the OBs.
The expectation was that UNO animals, lacking a normal path of odor access from the environment through an external naris to an intact central olfactory system, would have marked odor deficits compared to control mice.
Chamois and serow use their secretions from these glands to leave territorial olfactory signs and to scent-mark stems, bushes, and tree trunks as a dominance display during aggressive interactions with others of their species.
