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Honeybee eggs 0 6 hours old were collected from specifically designed hives kept in a flight room, allowing frequent collection of eggs of defined age with only slight disturbance of the colony [ 35, 36].
Wild individuals of BP (female, 1 year old), PM (female, 1 year old) and SH (female, 1 year old) were collected from Shenzhen Bay at Shenzhen and Qiao Island at Zhuhai, Guangdong Province, China in July of 2012 and PS (female, 1 year old) samples were collected in Malaysia.
The next morning, newly emerged bees (0 24 h old) were collected from the incubator and marked on the thorax with paint (Testors™) for identification.
During the same period, daily counts of hospital emergency department (ED) visits for asthma in children 2 18 years old were collected from all hospitals in the area.
Buccal samples (saliva) (18 donors; male and female; 26 60 yrs old) were collected from donors using sterile swabs by swabbing the inside of the donor's mouth.
Every 3 days, 600 newly eclosed callow workers, which were inferred to be approximately 0 1 days old, were collected from 8 10 stock colonies.
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Especially, regarding to the history of DTP vaccination, the vaccination coverage rate of sampling cities was recorded in local Center for Diseases Control and Prevention, and vaccination information for single child subject less than 7 years old was collected from certificates brought by parents or guardians.
Stool samples from 113 piglets up to 28-days-old were collected from 34 pig farms located in the States of Minas Gerais (MG), Mato Grosso do Sul (MS), Paraná (PR), Santa Catarina (SC), and Rio Grande do Sul (RS), during 2004 and 2005.
Forty 21-day old bees were collected from the hive, and briefly immobilized in a refrigerator.
For the injection of Drosophila embryos with bacterially purified Pgc (200 µM) in PBS containing 5% glycerol, 0 1 hour old embryos were collected from white1118 flies kept on apple juice agar at 19°C and 65 70% humidity.
Two-week old seedlings were collected from pots and washed with water to remove all soil.
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