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Later I looked out my old Sphere edition of French's iconic novel of 1977.
The whole adventure begins because Yuna believes she has seen Tidus in a really old sphere, but it turns out to be the spirit of a young man resembling him named Shuyin, who was the lover of a songstress and summoner named Lenne.
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8 weeks old spheres were disrupted into ∼0.1 mm diameter clusters by fine pipettation.
Strikingly, when 3 day old spheres were transferred to 3D collagen, ductal structures were formed (Fig. 2B) that expressed CK 14 (Fig. 2C).
Furthermore, human glands also expressed c-Kit exclusively in ductal cells (Fig. S4P,Q), and a subpopulation of these c-Kit+ cells could be isolated from 3 day old spheres by flow cytometry (data not shown).
Rat tail collagen (2.5 mg/mL, Roche, Indianapolis, MI; 1179179) was mixed with 3 day old spheres and cultured for seven additional days until branches and acinar-like outgrowth could be visualized.
Seven day old spheres were counted and analyzed using a GelCount™ automatic plate scanner (Oxford Optronics) and GelCount Version 0.025.1 software (Oxford Optronics).
The crew are able to track the Osaarian vessel, finding a large 1,000 year-old sphere constructed out of a single alloy.
(A ) Confocal images of 2-week-old spheres immunostained with KRT19, CD133, Ki-67, and Phospho-Histone H3 (all green).
Right panel: Average percentages (±S.E.M) of K5-expressing cells in 10- to 12-day-old spheres derived from untreated and HGF-treated Lu-pos cells.
The first generations of 10-day-old spheres were collected by gravity and dissociated to single-cell suspension with trypsin for serial passaging (G1 G3).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com