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To confirm the data obtained from 1D SDS-PAGE and 2D PAGE gel electrophoresis, a real-time RT-PCR was performed using total RNA extracted from mature potato tubers (two month old) of ten independent patatin-hpRNAi lines grown in greenhouse.
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Second, make it "older of two".
Mr. Chapman was the oldest of six children.
Radcliffe is the oldest of three boys.
I'm the oldest of six brothers.
I'M the oldest of six.
She was the oldest of five siblings.
The third oldest of eight kids.
He was the oldest of four children.
Paulin was the oldest of three boys.
I'M the older of two girls.
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CEO of Professional Science Editing for Scientists @ prosciediting.com