The Achilles tendons from 16 Sprague Dawley rats (weighing 200 250 g) around 7 8 weeks old were excised.
Antennae from gravid females (15 days old) were excised after emergence and stored at -20°C until use.
Walpole won the general election of 1734, which had given rise to many violent contests and a resurgence of the old bitterness about excise, but his growing unpopularity was underlined by the loss of many seats in the large seaports and heavily populated counties.
The nucleotide replacement happens before translation starts, and is mediated by various guide RNAs and enzymes that excise old and insert new nucleotides in a variety of ways to yield a product that is no longer complementary to the DNA stretch from which it was originally derived, and a protein that is no longer co-linear with the DNA sequence in the classical molecular biological sense.
Whole rosette leaves of 20 d old plants were excised, placed in a weighing boat and weighed at intervals for up to 9 h.
EDL muscles from the CON and STIM legs of young and old animals were excised and cut at mid-belly to obtain 2-3 mm serial sections.
Lung tissue from 6-week old animals was excised and assayed for glucose consumption and lactate production over 4 hr in ex vivo culture.
The distal halves of gametophore leaves of 3 weeks old plants were excised with a razor blade and cultivated on BCDAT medium for 4 5 days to analyse cell division and formation of chloronemal apical stem cells.
In brief, lungs of TIMP-1 gene-deficient and wild-type littermate mice (29 weeks old males) were excised and placed in Petri dishes containing 10 ml of medium (M199, Gibco, Invitrogen A/S, Denmark) supplemented with 30% foetal calf serum (FCS), penicillin (100 units/ml), streptomycin (100 μg/ml) and 1% NaHCO3 (Gibco, Invitrogen A/S, Denmark).
To examine cytokinin regulation, leaves from a 20 day old plant were excised and placed in water and gently shaken for 1 hour at which point either 5 μM cytokinin (benzyladenine) or the carrier solvent DMSO was added with shaking allowed to continue (after Rashotte et al., 2003[ 19]).
