Oil enables differentiation among degrees of reflective light, from shadow to bright beams, and minute depictions of light effects through the use of transparent glazes.
IVF was done in DMEM medium and after 4 h incubation, the eggs were washed to remove excess sperm and transferred to microdrops of 50 µl embryo culture medium (KSOM) containing 20 fertilized oocytes for an additional 24 h culture period under oil allowing differentiation to the morula stage.
After 2 weeks of differentiation, oil red O staining was used for detection of accumulated oil droplets.
At day 4 of differentiation, Oil Red O staining was used to visualize lipid droplets, which characterize mature adipocytes.
To demonstrate adipogenic differentiation, oil red O (Sigma-Aldrich) staining of lipid droplets was performed.
To assess the efficiency of adipogenic differentiation, Oil Red O-stained cells were extracted with 100 μl of 4%NP-40NP-40isopropanol for 5 min, and the absorbance of the dye triglyceride complex was measured at 520 nm.
Upon addition of adipogenic or osteogenic factors to MSCM (see Additional file 2E) or M5, hAMSCs differentiated into cells expressing adipocytes and osteocytes markers, respectively, as depicted by adipocyte differentiation-specific Oil red O and osteocyte differentiation-specific AP staining.
A differentiation between oil house paints and artists' oil tubes would be of interest in this context, but is not achievable by FTIR spectroscopy and requires micro-destructive mass spectrometry analysis [29].
Lipid vacuoles were detected in differentiated and controls bMSC after 18 days of adipogenic differentiation using oil Red staining.
As the maturity increases, or the migration and differentiation increased, crude oil density became low, and fluorescence colors of the petroleum inclusions captured in this stage are mainly white, yellow or yellow green.
