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At higher mass ratio oil:buffer (up to 30 wt.%) full hydrolysis could also be attained after 2 h at 37.5 °C.
An experimental design was performed to better understand the influence of the independent variables (mass ratio canola oil:buffer, temperature and CaCl2 concentration) and their interactions in the hydrolysis degree.
The main effects were fitted by multiple regression analysis to a quadratic model and full hydrolysis of canola oil was achieved at 37.5 °C without CaCl2 addition and mass ratio oil:buffer (22.1 wt.%).
A 24 full factorial central composite design was applied and the optimal conditions were found to be pH 7.0, enzyme concentration 7.42 mg/g oil, buffer concentration 0.20 g/g oil and agitation 1400 rpm with the maximum response of 76% in 4 h.
A 24 full factorial central composite design was used to develop the quadratic model that was subsequently optimized and the optimal conditions were as follows: temperature 40 °C, pH 7.72, enzyme concentration 5.28 mg/g oil, buffer concentration 1 g/g oil and there was 65.5% conversion in 6 h.
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The optimal conditions obtained on numerical optimization of this model were; temperature 24.2 °C, pH 8.70, enzyme concentration 2.71 mg/g oil and buffer concentration 1.25 g/g oil.
In this study, response surface methodology was applied to optimize process variables like temperature, pH, enzyme concentration (mg/g oil), and buffer concentration (g/g oil) for hydrolysis of castor oil using Candida rugosa lipase.
The mass m of the drop was calculated according to m = v*Δρ (v is the volume of the drop and Δρ is the volume mass difference between oil and buffer phases).
Higher oil prices buffered energy stocks, as China announced more loans-for-oil deals with trading partners.
(B ) Cholesterol decreases the stability of artificial oil microdroplets in buffer.
Being land based allows Pioneer to benefit from higher oil prices, but buffers it from the shutdowns and service interruptions that plague drillers on marine platforms.
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