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OH (see Additional File 1, Numerical simulation section).
They made this judgment by pressing a button and, using a pre-assigned hand-shape, either a CH vertical to the button or an OH (see Figure 1.A and B).
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The resin was washed and the product secondary amine acylated with addition of N-α-Fmoc-Gly-OH (see above) in DMF (6 mL) and incubation (1 h, 37 °C).
The phenolic proton possibly transfers through Asp84, which forms a weak H-bond with Tyr122-OH (see Figures 9 and 10) in the met-RNR structure (met = Fe1 III Fe2 III)).
Homostar 1 was first condensed with 4.5 equivalents 5′-Dmtr-2′-methyl-3′-succinyl uridine (2, Dmtr-mU-Suc-OH), see Scheme 1. Classical activation with 8 equivalents N, N′-diisopropyl carbodiimide, in addition to catalytic 4- dimethylamino pyridine (DMAP, 0.2 equiv) in THF, was incomplete with excess uridine succinate being consumed as the acyl urea.
Uh-oh See, you're getting it already.
Oh, seeing that never gets old!
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