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Then flux swing is provided by the coils from OH 1 to OH 7, while the stray field in the plasma region is minimized by the coils from OH 8 to OH 13.
The PCR products were resolved on 4.5% Super Fine Resolution (SFR) agarose (Amresco, Solon, OH), 1 × TBE gels containing ~ 2 μg/ml ethidium bromide.
Non-inonic contrast medium (Omnipaque; GE healthcare, Seoul, Republic of Korea) (850 mgl/kg) was i.v. injected with auto injector (CT9000; Liebel-Flarsheim, Cincinnati, OH) (1 ml/sec) for aortic and venous phase scanning.
The colon cancer cell line, Caco-2/15 was grown in DMEM (GIBCO, Burlington, ON) supplemented with 10% fetal bovine serum (ICN Biomedicals, Aurora, OH), 1% HEPES and 1% Glutamax (both from GIBCO, Burlington, ON) as described previously [ 23].
After addition of 75 μl of NaNO2 (5%), 150 μl of freshly prepared AlCl3 (10%), and 500 μl of Na OH (1 N), the volume was adjusted with distilled water until 2.5 ml.
Whole cell lysates were prepared by resuspending cells in RIPA buffer (50 mM Tris-HCI, pH 7.4, 150 mM NaCl, 1% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% SDS) containing 100 μg/ml PMSF (phenylmethylsulfonyl fluoride; USB Corporation, Cleveland, OH), 1 mM sodium orthovanadate and 20 μg/ml aprotinin (Sigma-Aldrich Company (St . Louis MO) on ice for 1 hour.
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The reaction is complicated but can be briefly expressed as the following equation [21]: 4 Au + 8 S 2 O 3 2 − + O 2 + 2 H 2 O → 4 Au S 2 O 3 2 3 − + 4 OH − (1).
The reaction for the growth of ZnO crystal may be simply expressed as follows: CH 2 6 N 4 + 4 H 2 O ↔ CH 2 6 N 4 − 4 H + + 4 OH − (1).
It has been suggested that the cause is residual species such as F and OH [1] and their chemical action with time [12, 14, 23].
Furthermore, PIP2-1 significantificantioxidantdactivityvity against hydroxyl radicals (OH), 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH), 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic
Furthermore, it showed stronger antioxidant activities compared with hot water extraction by evaluating in superoxide radical (O−2), hydroxyl radical (OH), 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical, reducing power and metal chelating assay.
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