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Regulatory networks are known to evolve quickly and both the DNA-binding domains of transcription factors and their target sequence sets are highly dynamic (i.e., orthologous regulators are often regulating non-orthologous targets), making comparative studies difficult [ 43, 44].
Through interactions with 3′UTRs, miRNAs can modulate the expression of many genes simultaneously, often regulating individual signaling pathways at multiple levels.
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Members of the multiple antibiotic resistance regulator (MarR) family often regulate gene activity by responding to a specific ligand.
Functionally related genes that are involved in the same biological pathways are often regulated by similar gene regulators (16, 17).
Alternative exons are often regulated according to cell-specific patterns and regulation is mediated by specific sets of cis-acting elements and trans-acting factors.
One of the origins of network structure is combinatorial regulation; i.e. single TFs often regulate more than one target, and particular targets are often regulated by more than one TF.
As with their regulation of each other, it is likely that they also often regulate these downstream target genes in a coordinated manner [17].
Transcription factors often regulate the expression of different cytokines and are essential for the regulation of gene expression.
Derivative uses are often regulated by intellectual property system.
Protein phosphorylation often regulates interactions between components of signalling networks.
These rhythms are often regulated by short exposure to light.
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