Since direct detection of target DNA in plant material with PCR-based methods often lack sensitivity, multiplication of the target cells in or on growth media prior to DNA detection is desirable.
Nursing home patients typically are afflicted with several comorbidities, including depression, declining functional status, chronic heart failure, impaired cognition, and respiratory ailments; thus clinical symptomology and laboratory results often lack sensitivity and specificity for NHAP [ 20].
Given that depressed mothers often lack sensitivity in interactions with their children [ 11, 12], children who have been exposed to early maternal depression can be hypothesized to be less ego-resilient later in life than those who have not.
Moreover, these assessment batteries and standardized tools rely on quantitative scales, which are often lacking sensitivity.
Modified Hodge test is the only test recommended by CLSI for the phenotypic detection of carbapenemase producers but often lacks sensitivity and specificity.
However, PCR amplification of O. tsutsugamushi DNA from blood often lacks sensitivity because some hemoglobin, iron porphyrin, and other factors may inhibit the PCR, although obtaining and processing the blood that avoids the inhibitors is possible (3, 4, 9 ).
However, they are not well correlated with cognitive deficits, and molecular imaging methods that can image them in vivo often lack the sensitivity and specificity for detecting AD in its earliest stages, when therapeutics may be most effective.
Diagnostic tests based on conventional or real-time PCR often lack the sensitivity required to detect low concentrations of pathogen DNA.
Recent research by McFadden, Renfrew and Atkin [ 34] and Puthussery et al. [ 35] in the UK, indicates that maternity services often lack cultural sensitivity and health professionals have a tendency to make assumptions about, or stereotype, women from ethnic minorities.
Although high-throughput, microarray-based assays have been developed to measure differences in mRNA expression among independent samples, these assays often lack the sensitivity to detect rare mRNAs and the reproducibility to quantify small changes in mRNA expression.
When used to compare mRNA expression among independent samples, microarray-based assays typically require large numbers of samples to obtain statistically significant correlations between genetic variants and mRNA expression and, in the absence of a cDNA PCR amplification step, often lack the sensitivity to detect rare mRNAs [ 16- 18].
