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Compounds having anions such as sulfide (S2−), hydroxide (OH−), carbonate (CO32−), and phosphate (PO43−) are often insoluble in water.
Damaged or misfolded proteins in aggresomes are often insoluble in non-denaturing detergents [ 4].
Unfortunately, shell matrix proteins are often insoluble, highly acidic or complexed with minerals, making their purification very difficult [ 31].
A point to note is that simple metal alkoxide complexes are often insoluble in their parent alcohol.
These aggregates are often insoluble in non-ionic detergent lysis buffers, such as 1% Triton X-100 (Kaganovich et al., 2008; Buchberger et al., 2010).
The action of these enzymes consequently results in the formation of covalently crosslinked, often insoluble supramolecular structures and has a well-established role in tissue homeostasis in many biological systems (Aeschlimann and Thomazy 2000; Lorand and Graham 2003; Iismaa et al. 2009).
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However, when the generation of obsolete proteins exceeds the capacity of the cell to degrade them, there is a progressive accumulation of damaged proteins, which often become insoluble over time and are potentially toxic to cells [9].
When genes encoding such proteins are expressed in invertebrate models, the proteins often form insoluble aggregates that are associated with cellular toxicity and tissue dysfunction.
In the "late stage," these propagators react again with free amino groups, and through oxidation, dehydration and cyclization reactions, they form yellow brown, often fluorescent, insoluble, irreversible compounds (usually called AG Es, which accumulate on long-lived proteins and cause damage.
To apply ink-jet printing technology, dye-type color filter materials are preferred to pigment-type compounds because dye color filters are cheaper and have an easy control of fabrication process and a superior chromatic property, whereas the ink-jet nozzle is often blocked by insoluble pigment particles.
Overexpression of transgenes in heterologous expression systems often results in insoluble and non-functional proteins due to misfolding or incorrect posttranslational modifications.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com