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For 18 of 21 trait combinations higher estimates of rA were calculated with the parent-offspring method (mean traits animal model: mean rA = 0.22±0.24 SD; parent-offspring model: mean rA = 0.37±0.22 SD; ZW = 3.39, N = 21, P<0.001).
An important point in the experiment was that the parents of treatment larvae had also been reared at the same temperature of 18°C to control for the possibility of non-genetic parental effects on offspring (see Methods).
Then the osptr3 and osptr9 homozygote mutants were identified from the offspring by PCR method and were use for phenotype observation.
7 The use of the random assortment of genes from parents to offspring provides one method for assessing the causal nature of some environmental exposures.
Observing a sire-segregation pattern is dependent on the dam being fixed for one of the alleles at the RAD marker because there is no means to differentiate between homozygous and heterozygous offspring using this method.
The female seahorse (gonad already matured) was returned to the male side of the tank where she paired and mated again (not necessarily with the original partners) six days after the male released his offspring (a modified method from Masonjones' and Lewis' investigation (Masonjones and Lewis, 2000)).
In this study, genomic loci (eg, genes or chromosomes) are first partitioned into haplotypes, defined as a consecutive strings of SNPs transmitted together from parents to offspring, using existing methods (for example, HapLink, the HapMap website.
Using this method, the offspring from crossings with female T5038 should be black and the offspring from crossings with Mendoza females should be wild type.
Temporal locality is also adopted to help generate offspring in the second method proposed.
As expected, the offspring of the former method were identical to the parents. .
The wide age range (7 to 22) of our sample of offspring made the first method particularly suitable.
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