Sentence examples for off-target products from inspiring English sources

Untranslated off-target products would be apparent at the RNA level only and would require methods such as rapid amplification of complementary DNA ends (RACE) to be detected.

Two out of three off-target trans-spliced products will be out of frame and degraded by nonsense-mediated decay; only off-target products that are translated could represent a problem.

The off-target product is the higher molecular weight species (lane 13) and represents mispriming of the reverse primer to a downstream site in exon 3. DOI: http://dx.doi.org/10.7554/eLife.07540.010 To quantify the relative abundance of the mprs16 linear, circular, and exon-containing lariat species, we performed qPCR on RNA from both wild-type and dbr1∆ yeast.

The use of high stringency PCR cycles encourages the efficient and specific amplification of the target products, while off-target PCR products are progressively diluted.

The company is starting off targeting product engineers, where having the correct documentation and up-to-date information is often times seen as more important than many teams.

These expression vectors may produce results that are due to characteristics of transcription in the minigene and, in some cases, generate large amounts of off-target splice products.

(F) T7E1 assays assessed mutagenesis at 2 predicted off-target sites; PCR products were used as a control.

Detection of a single band with the C-terminal tau antibody in FLAG-immunoprecipitated proteins indicates that translated potential off-target trans-spliced products are minimal.

For specificity control we confirmed that all immuno-precipitated DNAs showed no products with off-target control primers (not shown) and no precipitates with irrelevant control IgG.

Contrary to on-target exome data, off-target reads are unintentional by-products of the enrichment technologies and are thus subject to a mixture of sequencing biases that are highly variable and dependent on genomic context.

We describe procedures for isolating cells carrying the substitutions and for eliminating the products of secondary off-target events.