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X.G. assisted with off-target analysis.
Off-target analysis of sgRNA2b-induced human ES cells.
The whole genome sequence dataset used for off-target analysis can be accessed at EGA (EGAS00001003436).
Extended Data Fig. 6 Off-target analysis of human CD34+ HSPCs edited by SpCas9 RNP targeting BCL11A enhancer.
J.H.H. designed the research, performed PACE, characterized variants in bacteria, conducted human cell experiments, analysed data, performed off-target analysis and wrote the manuscript.
Off-target analysis was performed following a previous study with modifications (Liang et al., 2015).
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Our off target analysis is relevant for the sequence selection used in transgenic plants.
In addition, off-target event analysis revealed that none of the off-target mutations was introduced into the embryos.
The primers for off-target site analysis can be found in Table S3.
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