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For these measurements, the test organisms had to be taken off the medium.
Until recently, brands and businesses have written off the medium as a place for teenagers to film short, quirky, and often nonsensical short form videos.
The method relies on the fact that, at least in certain conditions, linearly polarized incident light is partially depolarized by the target, while backscattering off the medium conserves the polarization state.
This permits to reject the received signal component due to scattering off the medium (optical noise), and improve the performances (signal-to-optical-noise ratio, contrast, target visibility range and phase measurement accuracy) of the underwater imager.
After that, the medium and cell debris were removed by pipetting off the medium and 2 ml of fresh serum-containing medium was added to the remaining cells.
It is a long way off the medium rare I ask for.
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This pilot scheme was intended to assess the impact of a switch-off of the medium-wave signal, and how many listeners would be unable – or unwilling – to listen to the stations via other means.
After 7 to 21 days (depending on the growth rate of a particular isolate), approximately 5 to 10 mg of mycelia were scraped off of the medium and placed into 600 μl lyticase solution [1 M sorbitol, 100 mM EDTA, 200 U lyticase (Sigma-Aldrich, St . Louis MO), and 14 mM beta-mercaptoethanol], ground with a pestle, and incubated at 30°C and 500 rpm for 1 hour.
Either side of lunch, when Vaughan struggled to get the brake off, the medium-pacer bowled 10 overs for 13 runs.
Cells subjected to OFF regimen 2 were incubated in fresh medium for 2 h after the first bout of OFF before collection of the medium.
The drug was washed off by aspiration of the medium and rinsing with 10 m M phosphate-buffered saline (PBS) 2 h (Group 4), 4 h (Group 5), 6 h (Group 6) and 24 h (Group 7) after exposure.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com