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For these reasons, before proteomic analysis, 4 mL of urine were concentrated and desalted using 3 kDa MW-cut off filter devices (Millipore).
This cut off filter was applied to ensure we had complete confidence in the integrity of the sample DNA used in the final analysis.
Of particular importance is the observation that the OFF filter has "faster kinetics", i.e., its peaks are closer to time zero as compared to the ON filter.
This means that activation of the OFF filter affects spike probability with a shorter latency an observation that is of obvious importance for explaining the differences in latency for the flashed gratings.
Preparation of Methyl Heptosides 14: 23a The crude heptose 1 (<121.5 mmol) was taken up in MeOH (500 mL); a small amount of a white fine precipitate was filtered off (filter paper).
The mixtures of INA-NHS with protein were stirred for 1 2 h at room temperature, and low mass products removed with a 10,000 MW cut off filter.
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Serum samples were diluted 1 2 into reaction buffer and ultra-filtered through a 10,000 molecular weight cut-off filter.
During the irradiation, no cut-off filter was used.
A 300-W Xe lamp equipped with a 420-nm cut-off filter was used as a visible light source.
The photocatalytic properties of ZnO/TiO2/Au and ZnO/TiO2(N /Au composites were studied under illumination with Xe lamp equipped with a 400 nm cut-off filter.
Following agitation, 4 mL of the solution was transferred into the Amicon® device equipped with a 3 kDa molecular cut-off filter and centrifuged for 25 min.
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