sGC and rGC homologues were located by performing multiple BLASTP [ 77] searches with a cut off expectation value (E-value) of 10-7 aGenBankGenBank.
All statistically significant EST sequence hits were extracted and subsequently searched locally against the C. elegans proteome [ 86] using BLASTX with a cut off expectation of 10-7.
Using each C. elegans GC gene as a query sequence, we performed exhaustive TBLASTN [ 77] database searches with a cut off expectation value of 10-7 againsthehe nematode EST database NEMBASE [ 83] and the Caenorhabditis briggsae genome at Wormbase [ 84].
"Our earnings today were just off expectations, but further off what we expect from ourselves," Mr. Mudd said in a statement.
Once you've shaken off expectations of coherence or relevance, you can marvel at the scale of Fellini's folly.
Shares in the company closed down 15 per cent after it informed the stock market that full-year profits would no longer hit targets, and could be as much as £40m off expectations.
On March 18, Pakistan's president, Pervez Musharraf, gave a television interview in which he described a pitched battle between Pakistani soldiers and 400 to 500 militants and set off expectations that Al Qaeda's No. 2 leader, Ayman al-Zawahiri, had been surrounded.
At that point, Trudeau brushed off expectations that he would succeed outgoing Liberal leader Michael Ignatieff, saying: "Because of the history packaged into my name, a lot of people are turning to me in a way that … to be blunt, concerns me".
Former Columbia Journalism Review editor Suzanne Braun Levine takes this as her cue in a new book, How We Love Now, arguing that older women have more satisfying experiences of intimacy because we can shuck off expectations of femininity, niceness and acceptability, to be more honest about desire.
In reality, that's not far off expectations.
Despite the abundance of natural gas reserves in Malaysia coupled with serious government thrusts to promote cogeneration, its (cogeneration) development pace lags far off expectations.
