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We see spikes of very high coverage that correspond to amplicon ends overrepresented in sequencing [ 22].
These results suggest additional difficulties for evaluation and annotation of very high coverage depth De-Bruijn based assemblies.
Repetitive sequences in introns resulted in small regions of very high coverage, 10-1000 times above the median.
These methods are conservative or generate many false-positive results, particularly when traversing highly inter-connected (complex) regions of the graph or in regions of very high coverage.
An example of very high coverage was observed in the cluster that contained smooth muscle differentiation markers, in which 6/7 (86%) ortholog pairs were positive for a Serum Response Factor (SRF) motif.
In addition, a single 41719-bp contig was identified as representing the mtDNA on the basis of very high coverage and extensive sequence similarity to known yeast mitochondrial genomes.
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The advent of NGS technologies, with their capability for very high coverage, allows the analysis of mtDNA mutations at very low frequency with high accuracy [ 88].
Thus, the advent of next-generation sequencing technologies, with the potential for very high coverage, seems ideal for investigating heteroplasmic events in very old people [ 27].
Conversely, because of the very high coverage of the plastid genome, any plastid-copy reads that escaped repeat masking might introduce errors into the mitochondrial assembly at sites of plastid integration.
Read overlap due to this phenomenon inflated the coverage values, so many of the very high coverage values are likely artifacts of the unigene creation and masking process.
This targeting may lead to inequity by design, but within these designated areas, the approach of achieving very high coverage (i.e., >90% in the geographically targeted area) should maximize both coverage and equity for that population.
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