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Each probe gives rise to an amplification product of unique length separated by capillary sequencer (Genescan).
To evaluate the trade-offs between read length 'f' and HLA typing, we computed the number of unique length oligonucleotides in each HLA sequence.
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Genomic DNA was digested with Tsp509I giving rise to integration specific fragments of unique lengths.
This resulted in a total read length of 35,035,388 nucleotides and a total unique length of 10,420,310 nucleotides that were mapped to the assembly.
The downstream probe of MLPA has a stuffer sequence with variable length to generate the unique length of ligated probes, and these probes are too long to be chemically synthesized [ 9].
It is also difficult to rationalize, that if the unique length of M. burgessensis and P. zenobia chaetae evolved as effective sensory tools against predation, why haven't similar sized structures been employed in a similar manner by subsequent brachiopod lineages?
The number combinations of Amplicon X Unique Fragment Length Distributions is the number of unique fragment length distributions summed over all variable amplicons.
Through a read filter process, NGSmirPlant will obtain the unique length distribution of small RNA transcriptome obtained from deep sequencing, which can be used to distinguish the different classes of small RNAs and estimate the performance of sequencing library constructed.
The test, first developed in 1985, looks at four to six segments of DNA that have a unique length in each individual.
They contained 7500 amplification PCR products of unique full length cDNA clones from the Drosophila Gene collection release version 1.0 (Berkeley Drosophila Genome project).
The distribution of unique sRNA length is summarized in Figure 2. The overall distribution pattern was similar between WT and MT, i.e. the majority of sRNAs (approximately 95%) were 21-24 nt in length with 24-nt sRNA as the major peak.
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