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Thus, the difference Y pal (f) = f pal – f, where f pal is the fraction of underrepresented sites in actual palindrome dataset, is an estimation of the fraction of sites underrepresented due to R-M system presence in a genome.
χ test or exact Fisher test were used for comparison of fractions of underrepresented sites in datasets.
If so, the majority of underrepresented sites in the prokaryotic control dataset are traces of lost R-M systems.
Figure 3 demonstrates that fractions of underrepresented sites in actual palindromic and non-palindromic datasets nearly coincide.
We speculate that the majority of underrepresented sites could be traces of lost orthodox R-M systems.
Certainly, the threshold choice influences the fraction of underrepresented sites in the cohort of site-genome pairs under our study.
Similar(49)
Two additional loci, atpF and gap, were selected because they are housekeeping genes that have been used in other MLST schemes [29], [30], and are located at underrepresented sites of the B. quintana chromosome.
Most likely the majority of non-underrepresented sites of actual site-genome pairs are the sites of recently acquired R-M systems.
This observation confirms that a significant part of non-underrepresented sites of the orthodox R-M systems could be the sites of recently acquired R-M systems.
Percentages of site-genome pairs with the underrepresented sites for different R-M system types are shown in Table 2.
In other words, closely related genomes share similar underrepresented sites pattern regardless of encoded R-M systems.
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