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Determination of total protein was performed using biuret method.
The concentrations of total protein in the samples were detected and calculated from a standard curve.
Total luciferase activity was measured and values were normalized to amounts of total protein.
The concentration of total protein in BALF was used to assess pulmonary vascular leakage.
The largest proportion of total protein content was found in adults fed P. lutheri (33.75 ± 0.87 %).
40 μg of total protein was used for Western blot analysis based on standard procedures.
However, administration of the extracts resulted in significant decreases in the levels of total protein.
Equal amounts of total protein were loaded in lanes 1 2.
The amount of total protein was determined according to Lowry et al. (1951).
We determined the concentration of total protein in the BALF to assess pulmonary vascular leakage.
Five micrograms of total protein per lane was analysed by a 12%% SDS-PAGE.
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