Exact(2)
* Percentage of total monosaccharides; ND, non-detectable.
With regard to monosaccharide composition of hemicelluloses, we found that arabinose (Ara) and xylose (Xyl) covered more than 95% of total monosaccharides (Additional file 4), indicating that xylans are the major hemicelluloses in both wheat and rice plants.
Similar(58)
Figure 4 presents the cumulative effect of each enzyme combination on total monosaccharide conversion based on the theoretical maximum yield.
No differences were found in released oligosaccharide patterns between wild type and transgenic lines using ESI-MS (Additional file 6) or HPAEC-PAD (Additional file 7), similarly for total monosaccharide composition of released material (Additional file 8), for the XEG-insoluble fraction.
The RCA, PNA, and UEA datasets showed similar profiles (Fig. 8), whereas the separation between binding sites derived from the MALII dataset on pPGM was 2-fold greater (Table 2), as expected from the mucin chemical structure, where NeuAc represents only 1% of the total monosaccharide content (Supplemental Table S1 and ref. 28).
Ratios of LX3/(LX3+LX4) between 0.4-0.7 0.4-0.7 comparable total monosaccharides yielded
aNon-structural carbohydrates expressed as total monosaccharides.
In contrast, the share of oligosaccharides in the formic acid-ensiled hemp was lower, the difference between reducing sugars and total monosaccharides being only 11%to20%0%.
Transformants in the irx9 background were additionally subjected to analysis of total wall monosaccharide composition after hydrolysis with sulfuric acid, in order to calculate the ratio between total C6 and C5 sugars in the wall.
The total monosaccharide (glucose and fructose) content of GJ + tfosEp (73.31 [±0.67] mg mL−1) was over two-fold higher than that of untreated GJ (30.39 [±1.51] mg mL−1); the sucrose content of both was negligible (0.60 [±0.06] mg mL−1).
This strategy provides high total monosaccharide recovery or high glucose recovery from lignocellulosic materials, depending on the autohydrolysis conditions applied.
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