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FCCP per se induced the greater release of l-[14C]glutamate in hypergravity as compared to control (4.8 ± 1.0% and 8.0 ± 1.0% of total label).
Radiolabelling is a sensitive approach and allows determination of total label uptake since the total radiolabel content is easy to detect.
Depolarization stimulated release of cytosolic l-[14C]glutamate from synaptosomes preliminary treated with FCCP was considerably increased from 27.0 ± 2.2% of total label in control to 35.0 ± 2.3% in hypergravity.
Semiquantitative estimation of immunogold labeling on keratinocyte cryosections showed that caveolin-1 labeling localized predominantly at and in close vicinity to the plasma membrane of control cells (∼66% of total label peripheral, ∼33% intracellular), whereas ILK-K5 cells showed an increased intracellular caveolin-1 labeling (∼33% peripheral, ∼66% intracellular).
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Repressing FoxC2 activity with a dsRNA or with a dominant negative FOXC2-Engrailed fusion protein resulted in an increased proportion of GFP-labeled, desmin + myotomal cells out of total labeled cells, at the expense of SM.
The percentage of CFSE-positive erythrocytes was calculated as the percentage of total labeled fraction determined 6 hours after injection.
The differences of total labeling between samples (obtained by summing the volumes of 3D objects) reflected the differences in the distribution patterns or labeling levels.
The authors conclude that local chromosomal environment affects initiation rates by comparing different clonal cell lines that accrue different amounts of total labeled construct mRNA.
Fluorescence at the plasmalemma accounted for 66 70%, and cytoplasm for 30 34% of total labeling for Cntn4, -5 and -6 proteins (Fig. 1C).
When we compared between oral doses, the percentage of total labeled folate that appeared as unmodified folic acid in the hepatic portal vein was significantly greater (P < 0.0001) than the percentage of total labeled folate that appeared as unmodified 5-FormylTHF at each time point.
From Figure 2A, it can be calculated that, for the oral dose of folic acid, the AUCFolicdose of total labeled folate (folic acid + 5-MTHF) was 597 nmol · min−1 · L−1.
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