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Therefore the application of this assay is rather limited.
Identification for the limit of quantitation of this assay.
Standardization of this assay is a challenging task.
The detection limit of this assay was 102 copies.
The diagnostic performance of this assay was studied at two independent reference laboratories.
The sensitivity and specificity of this assay were 88.9% and 88.9% respectively (compared with quantitative cultures).
The main advantage of this assay is its simplicity and ease of use.
Practical use of this assay in tap water and human urine was successfully demonstrated.
The analytical specificity of this assay was successfully tested to identify L. brevis isolates from sourdoughs.
We demonstrated the utility of this assay in spiked human sputum specimens.
The sensitivity of this assay was compared with the malachite green method and bioluminescence method.
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