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There is a possibility of third chromosome dominant modifiers.
Major codons were identified by estimating the relative frequency of each synonymous codon encoding a particular amino acid across our set of third chromosome genes.
A higher fraction of third chromosome genes are differentially expressed between Y M and III M house fly males than genes on any other autosome (fig. 2 A).
However, a significant excess of third chromosome genes is also upregulated in III M males (supplementary fig. S3, Supplementary Material online), suggesting that the differential expression of third chromosome genes between Y M and III M males is not the result of degeneration of the III M chromosome.
One possibility is that the unique system of third chromosome inversions in this species has influenced the genetic diversity available within populations that enables them to respond effectively to environmental conditions [ 34].
Because of the large number of third chromosome gene arrangements in D. pseudoobscura, inversions not included in this study may potentially impact the estimation of the population-scaled recombination rate.
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Toward this end, we obtained a series of second chromosome Gal-4 lines that are differentially expressed in the various subsets of PDF neurons.
Recent studies have reported that dSETDB1 is specifically localized to the entire fourth chromosome in the polytene chromosome spreads of the salivary glands [26], [27], which prompted us to immunostain the germarium for the painting of fourth chromosome (POF) [35] that is known to coat the entire fourth chromosome.
Ki-67 nuclear antigen is a non-histone nucleoprotein in proliferating cells' nucliues and is closely linked to cell proliferation at region of tenth chromosome [ 70] in human genome.
Here, we established a number of second-chromosome substitution strains derived from a single Pennsylvania population (except strain CS) to evaluate CNV occurrence in completely homozygous genotypes.
Although previous CNV studies focused on heterogeneous strains, here, we established a number of second-chromosome substitution lines to uncover CNV characteristics when homozygous.
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