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Imperfect sensitivity of these swabs further adds to the uncertainty.
Six hundred forty-seven of these swabs were processed immediately after collection and 348 were received by mail.
The testing of these swabs has been considered by the Queensland University of Technology QUT Animal Ethics Committeeee and approved under Tissue Use Notification #1100000362.
All but one of these swabs were positive for at least one pathogen, and 18/27 (67%) were positive for all three otitis media pathogens-see Table 4.
A total of 300 swabs was taken from 100 pregnant women at 35-37 weeks of gestation, and 49 of these swabs were positive for a total of 22 subjects.
A cohort of 20 children born in 2007/2008 were sampled every month until two years of age, and the 16S rRNA gene profiles of these swabs were sequenced by 454 pyrosequencing.
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Of these, swab specimens were collected from 9 (90%) and blood samples from 7 (70%).
The testing of these swab samples, collected as a part of routine diagnostic investigations of koalas and livestock, has been considered by the University of Sunshine Coast USC Animal Ethics Committeeee and the requirement for ethics approval was waived (AN/E/14/01 and AN/E/14/02).
Of all these swabs, 302 were qPCR positive with an average Ct value of 28.09.
PCR was performed on enrollment oral swab samples from six participants in whom the corresponding plasma sample was PCR positive, but DENV RNA could not be amplified from any of these oral swabs so we did not continue with PCR on the remaining oral swab samples.
If a patient was considered part of a K. pneumoniae case cluster, a set of these surveillance swabs was taken from the patient's incubator or bed.
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