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Candidate RAD alleles displaying the inverse of these segregation patterns were also included.
As a result of these segregation defects ATMIN-deficient cells showed enhanced in micronuclei formation, an indication of chromosomal instability (Fig. 5D).
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Analysis of the segregation of these variants in a reciprocal F1 cross, as well as genotyping two doubled haploids and their diploid parents, reveals both allelic (segregating) and widespread paralogous (fixed) sequence differences.
In 2010, Biggins and her colleagues were the first to purify kinetochores and, using this system, have already made several groundbreaking discoveries about the function and structure of these crucial components of the segregation machinery.
While some increase in segregation efficiency of plasmids is derived from having either one of these elements, high segregation efficiencies were only achieved when both the PCEN repeat and core regions are present, indicating that the entire A/T-rich sequence (i.e., both the core and the repetitive regions) is required for functioning of a centromere.
Rather, the distinction on the basis of reproductive possibilities shapes and is shaped by the sorts of cultural and social phenomena (like varieties of social segregation) these possibilities gives rise to.
All except two of these also show segregation distortion.
Most of these loci with segregation distortion were mapped on linkage groups b08f, b03c or b02d.
We jointly inferred the phases of these SNPs and segregation pattern (offspring genotypes) in the mapping cross for each marker in a maximum likelihood framework (Methods).
Ubiquitination of the mitofusins appear to be of particular relevance as degradation of these proteins insures segregation of dysfunctional mitochondria, as there is no functional mechanism to tether adjacent mitochondria for fusion events.
In most of these cases, the segregation of resistance suggested that several loci might contribute to the trait, but the loci tagged by the markers, that now correspond to contigs rich in NBS-LRRs, accounted for the largest effect [ 53].
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