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Both of these differentiation antigens are expressed on >90% of melanomas [46].
Therefore, we decided to test this hypothesis by focusing on defined stages of these differentiation programs.
It cannot be excluded that HP1γ contributes to the repression of these differentiation genes.
The original definition of these differentiation stages is derived from the measurement of CCR7 and CD45RO [33]; however, others have used CD27 and CD45RO to assign these stages [32], [35].
We only detected procyclin in PCFs and PAD1 was exclusively detected in stumpy cells, suggesting that knockout of RabX1 or RabX2 did not lead to expression of either of these differentiation markers (Figure 10B).
Elucidating the cellular and molecular mechanisms that regulate initiation and termination of the expression of these differentiation factors will be important for understanding the developmental programs in these tissues.
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Chromatin immunoprecipitation demonstrated that Brn2 bound to the promoter regions of these differentiation-related genes.
Many of the earlier phase genes that are later down-regulated are involved in B cell development, indicating that sustained Myc activation leads to the silencing of these differentiation-related genes in favor of those that drive cells into cycle with a sustained proliferative program.
Of note, these differentiation markers were not expressed in the NS cells.
One of these is differentiation and the other is integration.
Most of these classical differentiation genes were found to have orthologs in K. setae.
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CEO of Professional Science Editing for Scientists @ prosciediting.com